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structured gene中文是什么意思

  • 结构基因

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  • Expression of human papillomavirus type 16 l1 structure gene in sf9 cells and its products assembling into virus - like particles
    1在昆虫细胞中的表达及其生物学活性
  • Now , the structure gene sequence and 3 ' - end gene sequence have been logged on genbank . the accession number is ay044918
    目前,此过敏蛋白tb22kda的结构基因与3 ’端基因序列已在genbank登录,登录号为ay044918 。
  • The promoter and structure genes of x phage lysis genes were amplified by pcr , respectively . the plasmid mvps was constructed for integrating the vhb gene and the lysis genes into the chromosome of e . colihms174 by ligating the lysis genes to mini - tn5
    利用pcr扩增得到噬菌体裂解基因的启动子和结构基因( srrz ) ,连接在转座子minitn5上,构建成为质粒mvps ,用于将vhb基因和srrz基因共同插入到e . colihms174的染色体上。
  • Technical features : adopt new technology fabrics seven results , with mature ceramic production technology , powder buwen various raw materials to be transparent , according to the natural stone structure genes , generating component fabrics , rich layers . natural stone texture stronger products
    技术特点:采用全新七级布料科技成果,结合成熟的陶瓷生产工艺技术,以各种粉料布纹加以透明原料,根据天然石材的构造基因工程,生成通体布料、层次丰富、天然石材质感更强产品
  • According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat , we designed primers and got the structure gene successfully . by 3 ' - race method combined with nested pcr , the 3 ' - end nuclear acid sequence was also obtained ; in additon , for the 5 ' - end sequence , we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer , and till now , partial 5 ' - end sequence has been amplified as well
    本研究根据先前分离纯化所得天然tb22kda蛋白经maldi - tof - ms (质谱法)测得的氨基酸序列和文献报道的过敏蛋白核苷酸序列设计引物,扩增克隆了该过敏蛋白结构基因的编码序列;根据测得的序列设计特异性引物,并利用3 ’ - race方法结合巢式pcr扩增得到基因的3 ’末端;依据同源性比较的结果选用一段保守序列为5 ’引物,并根据结构基因内部序列设计3 ’特异性引物,进一步获得了该基因5 ’端的部分序列。
  • We paid our main attention to cloning tb22kda gene from tartary buckwheat , expressing the structure gene and getting the purified recombinant protein , and these provide foundation for father study on the relationship between structure and function of the allergenic protein tb22kda , the orientation of the corresponding epitope and the exploration of allergenic reaction mechanism
    本研究的目的在于分离克隆苦荞中的主要过敏蛋白( tb22kda )基因,并表达纯化出其结构基因编码的蛋白。从而为进一步研究过敏蛋白tb22kda的结构与功能,寻找其中的抗原决定簇,探讨过敏原与其相应抗体相互作用机理提供依据。
  • A new e . coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1 , bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed . 2 , a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter . 3 , another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19
    进一步以红移且荧光强度提高21倍的gfpmut3为报告基因,构建了大肠杆菌启动子探针载体phn1005 ,该载体上gfpmut3结构基因5 ’端的bamhi位点可用来克隆具有启动子活性的dna片段并定量分析插入的启动子强度;其3 ’端含rrnat1t2终止子,可允许克隆强启动子;在bamhi上游同样插入rrnat1t2终止子以防止载体puc19上的启动子的转录通读; gfpmut3结构基因上游还插入一段内含子序列使正反六种读码框的翻译均可被终止,可保证gfpmut3以正确的读码框翻译。
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Last modified time:Sat, 16 Aug 2025 00:29:56 GMT

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